Topic:
ผลงานและผู้ร่วม : SurasakYooyongsatit, KriangsakRuchusatsawat, NopadonNoppakun, NattiyaHirankarn, ApiwatMutirangura,JongkonneeWongpiyabovorn.
Abstract:
Alterations in LINE-1 methylation are related to many diseases. The levels and patterns of LINE-1 hypomethylation were
associated with a higher risk in developing several cancers, having a poorer prognosis and more aggressiveness. To evaluate the
LINE-methylated status in psoriasis, LINE-1 methylation in various cells from patients with psoriasis, squamous cell carcinoma
and normal controls were assessed by combined bisulfite restriction analysis of LINE-1. The results of the epigenetic changes for
intragenic LINE-1 gene expression were also tested on two known expression microarrays. In patients with psoriasis,
hypomethylation of LINE-1 and increase in %uCuC were prominent in the keratinocytes when compared with normal controls
(P=0.014 and P=0.020, respectively). Alternatively, %uCmC was significantly lower in patients with severe psoriasis compared
with mild psoriasis (P=0.022). The receiver-operating characteristic curve analysis indicated the high specificity and sensitivity
of uCuC and uCmC in detecting psoriasis and severity of psoriasis. From expression array analysis, genes with LINE-1 were
downregulated more than those genes without LINE-1 (P=3.84 × 10− 27 and P=2.14 × 10− 21, respectively). Modification in
LINE-1 methylation may alter the gene expression resulting in a phenotypic change of the psoriatic skin. %uCuC and %uCmC
may be used as biomarkers for psoriasis.